解析フロー



検体条件
clonoSEQ
- IDサンプル : クローン多様性を決定し、クローン特異的BCR遺伝子配列を同定する検体
- MRDサンプル: IDサンプルで同定されたBCR遺伝子配列を追跡するフォローアップ検体
IDサンプル | MRDサンプル | |
---|---|---|
gDNA 検体量 | 1μg以上 | 25μg以上 |
gDNA 検体濃度 | 15ng/μL以上 | 300ng/μL以上 |
- gDNA以外の各種検体も受け入れ可能です。詳細は、パンフレット「サンプルおよび返却データ」をご覧いただくか、お問い合わせください。
- 記載の量にはQCテスト分も含みます。
- MRDテストにおいて、95%の信頼度で10
-6 の感度(100万個の正常細胞中に1個の腫瘍細胞)を得るには、20μg 以上のDNAが必要です。

納品物
- 解析報告書
- 解析データ(CSVファイル):
- Assay Report(検体と解析結果に関する重要な情報)
- Clonality Report (識別されたドミナントシーケンス情報)
- Tracking Report(追跡されたシーケンス情報)

研究論文集
clonoSEQ
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Dimopoulos MA., et al. Daratumumab, lenalidomide, and dexamethasone for multiple myeloma. New England Journal of Medicine (2016)
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Wood B., et al. Measurable residual disease detection by high throughput sequencing improves risk stratification for pediatric B-ALL. Blood (2018)
Sala Torra O., et al. Next-generation sequencing in adult B cell acute lymphoblastic leukemia patients. Biology of Blood and Marrow Transplantation (2017)
Ladetto M., et al. Next-generation sequencing and real-time quantitative PCR for minimal residual disease detection in B-cell disorders. Leukemia (2014)
Faham M., et al. Deep-sequencing approach for minimal residual disease detection in acute lymphoblastic leukemia. Blood (2012)
Gawad C., et al. Massive evolution of the immunoglobulin heavy chain locus in children with b precursor acute lymphoblastic leukemia. Blood (2012)
Thompson PA., et al. Minimal residual disease undetectable by next-generation sequencing predicts improved outcome in CLL after chemoimmunotherapy. Blood (2019)
Brown JR., et al. Durable remissions with obinutuzumab-based chemoimmunotherapy: long-term follow-up of the phase 1b GALTON trial in CLL. Blood (2019)
Aw A., et al. Minimal residual disease detected by immunoglobulin sequencing predicts CLL relapse more effectively than flow cytometry. Leukemia & Lymphoma (2018)
Turtle CJ., et al. Durable molecular remissions in chronic lymphocytic leukemia treated with CD19-specific chimeric antigen receptor–modified T cells after failure of ibrutinib. Journal of Clinical Oncology (2017)
Ryan CE., et al. Ibrutinib efficacy and tolerability in patients with relapsed chronic lymphocytic leukemia following allogeneic HCT. Blood (2016)
Rawstron AC., et al. A complementary role of multiparameter flow-cytometry and high-throughput sequencing for minimal residual disease detection in chronic lymphocytic leukemia: an European Research Initiative on CLL study. Leukemia (2016)
Kriangkum J., et al. Single-cell analysis and next-generation immuno-sequencing show that multiple clones persist in patients with chronic lymphocytic leukemia. PLoS ONE (2015)
Wang M., et al. Durable response with single-agent acalabrutinib in patients with relapsed or refractory mantle cell lymphoma. Leukemia (2019)
Ruan J., et al. Five-year follow-up of lenalidomide plus rituximab as initial treatment of mantle cell lymphoma. Blood (2018)
Chen RW., et al. RB but not R-HCVAD is a feasible induction regimen prior to auto-HCT in frontline MCL: results of SWOG Study S1106. British Journal of Haematology (2017)
Armand P., et al. A phase 2 study of rituximab-bendamustine and rituximab-cytarabine for transplant-eligible patients with mantle cell lymphoma. British Journal of Haematology (2016)
Ladetto M., et al. Next-generation sequencing and real-time quantitative PCR for minimal residual disease detection in B-cell disorders. Leukemia (2014)
