Olink™ Proteome Analysis
Olink:
offering the capability to analyze 5,400 distinct proteins
Olink, developed by Olink Proteomics, revolutionizes protein analysis by combining highly specific Proximity Extension Assay (PEA) technology with next-generation sequencing. This groundbreaking platform enables the simultaneous quantification of up to 5,400 distinct proteins. This cutting-edge approach enables unparalleled sensitivity and high-throughput quantification, allowing researchers to uncover unprecedented insights into proteomics with exceptional precision.
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– Olink, part of Thermo Fisher Scientific
Our advantages of Olink Proteome Analysis
- Certified Service Provider: Riken Genisis is an Olink Certification Service Provider.
- High-throughput Capability: Our assay system is ideal for cohort studies involving thousands of samples.
- Comprehensive Biomarker Support: We offer identification and measurement services for drug development with recognized quality assurance, and have extensive experience with specimen types beyond serum and plasma.
- Proprietary Analysis Software: Riken genesis Visualizer for Proteome allows for intuitive and detailed data viewing.
- Advanced Data Analysis: We utilize Hitachi's proprietary AI, "B3," for advanced data analysis.
- Expert Support: Our experienced staff provides reliable service and support.
Special Services
Olink Explore 3072 Limited
While 88 samples are normally processed in one batch, with this service 22 samples can be analyzed in one batch for phase-in analysis.
Olink Explore HT Limited
While 172 samples are normally processed in one batch, with this service 86 samples can be analyzed in one batch for phase-in analysis.
Target specimens include plasma, serum, and cerebrospinal fluid. This service is especially recommended for those new to Olink analysis or those with a small number of specimens. It is also available as a pilot study.
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Olink Features
Highly Sensitive Quantitative Protein Analysis
By using PEA, which combines antibody and DNA-based methods, Olink has successfully eliminated antibody cross-reactivity and achieved high specificity, high sensitivity, and reproducibility.
What is PEA？
Using the PEA method, an immunoassay is performed using two specific single-stranded DNA-tagged antibodies against the target protein. When the two specific antibodies correctly recognize the target, double-stranded DNA is formed from the single-stranded DNA attached to each antibody. Next, PCR is performed using this double-stranded DNA as a template. Finally, the amplified DNA fragments are detected through real-time PCR or next-generation sequencing, and the expression level of the target protein is quantitatively analyzed.
Detection of Low-concentration Proteins in Blood
PEA technology uses DNA-tagged antibodies to achieve higher specificity than conventional methods. In addition, the amplification of DNA fragments by PCR increases detection sensitivity. As a result, PEA technology can detect low-concentration proteins, cytokines, and chemokines, which have been difficult to measure using mass spectrometry. Proteins at high concentrations are quantified using sample dilution rates optimized for antigen-antibody reactions.
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Olink Explore Series
The Olink Explore series offers two platforms: Olink Explore 3072 and Olink Explore HT.
Olink Explore 3072 can simultaneously detect up to 3,000 different proteins by using eight different panels (two for each of four categories: cardiovascular, cancer, neurology, and inflammation). Olink Explore HT consists of a single panel.
Summary of Proteins to be Measured
Secreted Proteins in Blood: Selected from the Blood Atlas
Tissue-specific Blood Biomarkers: Leaky proteins from 27 major organs
Inflammatory Proteins: Identified through text mining of 5,000,000 PubMed abstracts
Drug Target Proteins: Existing drugs and ongoing clinical trial candidates
Blood Proteins: Identified by mass spectrometry in blood
Protein list of Olink Explore 3072
Protein list of Olink Explore HT
Olink Explore 3072 Panel
横にスクロールしてご覧いただけます。
| Cardiometabolic Panels | Olink™Explore 384 Cardiometabolic Olink™Explore 384 CardiometabolicⅡ |
|---|---|
| Inflammation Panels | Olink™Explore 384 Inflammation Olink™Explore 384 InflammationⅡ |
| Neurology Panels | Olink™Explore 384 Neurology Olink™Explore 384 NeurologyⅡ |
| Oncology Panels | Olink™Explore 384 Oncology Olink™Explore 384 OncologyⅡ |
Data Analysis (Optional service)
Olink data can be analyzed in a variety of ways depending on the options selected.
Statistical analysis can be used to compare protein expression between groups, extract proteins with high expression variability, clustering, pathway analysis, and data visualization (scatter plots, clustering, etc.).
Example 1
Volcano Plot of Protein Expression Analysis
Example 2
Clustering of Protein Expression Analysis + Heat Map
Analysis Example 3
Pathway Analysis
Pathway enrichment analysis is performed using the GSEA tool.
Analysis Example 4
Riken genesis Visualizer for Proteome
Analysis results can be easily viewed using the Riken genesis Visualizer for Proteome.
For inquiries and quotes regarding Olink proteome analysis, Riken genesis Visualizer for Proteome and AI-based data analysis, please contact us.
Our Exceptional Quality
As a certified Olink service provider, RIKEN GENESIS is recognized for its reliability and expertise in delivering top-notch analysis. We are one of the few companies in the Asia Pacific region to hold this prestigious certification, underscoring our commitment to excellence in proteome analysis.
Quality Assurance
RIKEN GENESIS follows the standards for conducting genetic analysis studies in accordance with the Ministerial Ordinance on Standards for Conducting Non-clinical Studies on the Safety of Pharmaceutical Products. In addition, we are ISO 15189 certified and have received quality certifications from many organizations.
Sample Information
Sample Type
Serum or plasma or CSF
(For other sample types, please contact us.)
Deliverables
NPX data file (.csv)
An NPX data file (.csv) can be converted to an easily viewable form in Excel
Analysis Report (including Analysis Report output from OlinkTM NPX Explore)
Encrypted DVD-R, encrypted USB flash drive or encrypted HDD [including NPX file (.csv) and (in case of randomization) sample information file (.csv)
Special Services
Olink Explore 3072 Limited
While 88 samples are normally processed in one batch, with this service 22 samples can be analyzed in one batch for phase-in analysis.
Olink Explore HT Limited
While 172 samples are normally processed in one batch, with this service 86 samples can be analyzed in one batch for phase-in analysis.
Target specimens include plasma, serum, and cerebrospinal fluid. This service is especially recommended for those new to Olink analysis or those with a small number of specimens. It is also available as a pilot study.
– Contact us